diff --git a/libs/libc/misc/lib_mutex.c b/libs/libc/misc/lib_mutex.c
index 2518f3022d..379c812d06 100644
--- a/libs/libc/misc/lib_mutex.c
+++ b/libs/libc/misc/lib_mutex.c
@@ -241,7 +241,6 @@ int nxmutex_trylock(FAR mutex_t *mutex)
 {
   int ret;
 
-  DEBUGASSERT(!nxmutex_is_hold(mutex));
   ret = nxsem_trywait(&mutex->sem);
   if (ret < 0)
     {