diff --git a/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/#defconfig# b/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/#defconfig#
new file mode 100644
index 0000000000..ba94923f38
--- /dev/null
+++ b/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/#defconfig#
@@ -0,0 +1,44 @@
+#
+# This file is autogenerated: PLEASE DO NOT EDIT IT.
+#
+# You can use "make menuconfig" to make any modifications to the installed .config file.
+# You can then do "make savedefconfig" to generate a new defconfig file that includes your
+# modifications.
+#
+# CONFIG_NSH_DISABLE_IFCONFIG is not set
+# CONFIG_NSH_DISABLE_PS is not set
+# CONFIG_STANDARD_SERIAL is not set
+CONFIG_ARCH="arm"
+CONFIG_ARCH_BOARD="nrf5340-dk"
+CONFIG_ARCH_BOARD_NRF5340_DK=y
+CONFIG_ARCH_CHIP="nrf53"
+CONFIG_ARCH_CHIP_NRF5340=y
+CONFIG_ARCH_CHIP_NRF5340_CPUAPP=y
+CONFIG_ARCH_CHIP_NRF53=y
+CONFIG_ARCH_STACKDUMP=y
+CONFIG_ARCH_STDARG_H=y
+CONFIG_BOARD_LOOPSPERMSEC=5500
+CONFIG_EXPERIMENTAL=y
+CONFIG_FS_LITTLEFS=y
+CONFIG_INIT_ENTRYPOINT="nsh_main"
+CONFIG_MM_REGIONS=2
+CONFIG_MTD=y
+CONFIG_MTD_MX25RXX=y
+CONFIG_NRF52_QSPI=y
+CONFIG_NRF53_UART0=y
+CONFIG_NSH_ARCHINIT=y
+CONFIG_NSH_FILEIOSIZE=512
+CONFIG_NSH_LINELEN=64
+CONFIG_NSH_READLINE=y
+CONFIG_PREALLOC_TIMERS=4
+CONFIG_RAM_SIZE=524288
+CONFIG_RAM_START=0x20000000
+CONFIG_RAW_BINARY=y
+CONFIG_RR_INTERVAL=200
+CONFIG_SCHED_WAITPID=y
+CONFIG_START_DAY=26
+CONFIG_START_MONTH=3
+CONFIG_SYMTAB_ORDEREDBYNAME=y
+CONFIG_SYSTEM_NSH=y
+CONFIG_TASK_NAME_SIZE=0
+CONFIG_UART0_SERIAL_CONSOLE=y
diff --git a/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/defconfig b/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/defconfig
new file mode 100644
index 0000000000..5ff9c59011
--- /dev/null
+++ b/boards/arm/nrf53/nrf5340-dk/configs/qspi_cpuapp/defconfig
@@ -0,0 +1,50 @@
+#
+# This file is autogenerated: PLEASE DO NOT EDIT IT.
+#
+# You can use "make menuconfig" to make any modifications to the installed .config file.
+# You can then do "make savedefconfig" to generate a new defconfig file that includes your
+# modifications.
+#
+# CONFIG_NSH_DISABLE_IFCONFIG is not set
+# CONFIG_NSH_DISABLE_PS is not set
+# CONFIG_STANDARD_SERIAL is not set
+CONFIG_ARCH="arm"
+CONFIG_ARCH_BOARD="nrf5340-dk"
+CONFIG_ARCH_BOARD_NRF5340_DK=y
+CONFIG_ARCH_CHIP="nrf53"
+CONFIG_ARCH_CHIP_NRF5340=y
+CONFIG_ARCH_CHIP_NRF5340_CPUAPP=y
+CONFIG_ARCH_CHIP_NRF53=y
+CONFIG_ARCH_STACKDUMP=y
+CONFIG_ARCH_STDARG_H=y
+CONFIG_BOARD_LOOPSPERMSEC=5500
+CONFIG_DEBUG_FULLOPT=y
+CONFIG_DEBUG_SYMBOLS=y
+CONFIG_EXPERIMENTAL=y
+CONFIG_FS_LITTLEFS=y
+CONFIG_INIT_ENTRYPOINT="nsh_main"
+CONFIG_MM_REGIONS=2
+CONFIG_MTD=y
+CONFIG_MTD_MX25RXX=y
+CONFIG_MX25RXX_QSPI_FREQUENCY=98000000
+CONFIG_MX25RXX_QSPI_READ_FREQUENCY=98000000
+CONFIG_NRF53_QSPI=y
+CONFIG_NRF53_QSPI_RXDELAY=1
+CONFIG_NRF53_UART0=y
+CONFIG_NRF53_USE_HFCLK192M=y
+CONFIG_NSH_ARCHINIT=y
+CONFIG_NSH_FILEIOSIZE=512
+CONFIG_NSH_LINELEN=64
+CONFIG_NSH_READLINE=y
+CONFIG_PREALLOC_TIMERS=4
+CONFIG_RAM_SIZE=524288
+CONFIG_RAM_START=0x20000000
+CONFIG_RAW_BINARY=y
+CONFIG_RR_INTERVAL=200
+CONFIG_SCHED_WAITPID=y
+CONFIG_START_DAY=26
+CONFIG_START_MONTH=3
+CONFIG_SYMTAB_ORDEREDBYNAME=y
+CONFIG_SYSTEM_NSH=y
+CONFIG_TASK_NAME_SIZE=0
+CONFIG_UART0_SERIAL_CONSOLE=y
diff --git a/boards/arm/nrf53/nrf5340-dk/include/board.h b/boards/arm/nrf53/nrf5340-dk/include/board.h
index 5ee9b31916..e535d03f0a 100644
--- a/boards/arm/nrf53/nrf5340-dk/include/board.h
+++ b/boards/arm/nrf53/nrf5340-dk/include/board.h
@@ -155,4 +155,28 @@
 #define NRF53_ADC_CH2_PIN (GPIO_MCUSEL_APP | GPIO_INPUT | GPIO_PORT0 | GPIO_PIN(6))
 #define NRF53_ADC_CH3_PIN (GPIO_MCUSEL_APP | GPIO_INPUT | GPIO_PORT0 | GPIO_PIN(7))
 
+/* QSPI Pins ****************************************************************/
+
+/* QSPI0
+ *   QSPI CS  - P0.18
+ *   QSPI SCK - P0.17
+ *   QSPI IO0 - P0.13
+ *   QSPI IO1 - P0.14
+ *   QSPI IO2 - P0.15
+ *   QSPI IO3 - P0.16
+ */
+
+#define NRF53_QSPI0_CSN_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_VALUE_ONE | GPIO_PORT0 | GPIO_PIN(18))
+#define NRF53_QSPI0_SCK_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_PORT0 | GPIO_PIN(17))
+#define NRF53_QSPI0_IO0_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_PORT0 | GPIO_PIN(13))
+#define NRF53_QSPI0_IO1_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_PORT0 | GPIO_PIN(14))
+#define NRF53_QSPI0_IO2_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_PORT0 | GPIO_PIN(15))
+#define NRF53_QSPI0_IO3_PIN (GPIO_MCUSEL_PERIP | GPIO_OUTPUT | \
+                             GPIO_PORT0 | GPIO_PIN(16))
+
 #endif /* __BOARDS_ARM_NRF53_NRF5340_DK_INCLUDE_BOARD_H */
diff --git a/boards/arm/nrf53/nrf5340-dk/src/Make.defs b/boards/arm/nrf53/nrf5340-dk/src/Make.defs
index d28956f307..3ce6ef9935 100644
--- a/boards/arm/nrf53/nrf5340-dk/src/Make.defs
+++ b/boards/arm/nrf53/nrf5340-dk/src/Make.defs
@@ -56,6 +56,10 @@ ifeq ($(CONFIG_USBDEV_COMPOSITE),y)
 CSRCS += nrf53_composite.c
 endif
 
+ifeq ($(CONFIG_NRF53_QSPI),y)
+CSRCS += nrf53_mx25.c
+endif
+
 DEPPATH += --dep-path board
 VPATH += :board
-CFLAGS += $(shell $(INCDIR) "$(CC)" $(TOPDIR)$(DELIM)arch$(DELIM)$(CONFIG_ARCH)$(DELIM)src$(DELIM)board$(DELIM)board)
\ No newline at end of file
+CFLAGS += $(shell $(INCDIR) "$(CC)" $(TOPDIR)$(DELIM)arch$(DELIM)$(CONFIG_ARCH)$(DELIM)src$(DELIM)board$(DELIM)board)
diff --git a/boards/arm/nrf53/nrf5340-dk/src/nrf5340-dk.h b/boards/arm/nrf53/nrf5340-dk/src/nrf5340-dk.h
index 4b0b0ecef7..0b52d1d2a2 100644
--- a/boards/arm/nrf53/nrf5340-dk/src/nrf5340-dk.h
+++ b/boards/arm/nrf53/nrf5340-dk/src/nrf5340-dk.h
@@ -118,5 +118,17 @@ int nrf53_pwm_setup(void);
 int nrf53_adc_setup(void);
 #endif
 
+/****************************************************************************
+ * Name: nrf53_mx25_initialize
+ *
+ * Description:
+ *   Initialize the MX25RXX QSPI memeory
+ *
+ ****************************************************************************/
+
+#ifdef CONFIG_NRF53_QSPI
+int nrf53_mx25_initialize(void);
+#endif
+
 #endif /* __ASSEMBLY__ */
 #endif /* __BOARDS_ARM_NRF53_NRF5340_DK_SRC_NRF5340_DK_H */
diff --git a/boards/arm/nrf53/nrf5340-dk/src/nrf53_bringup.c b/boards/arm/nrf53/nrf5340-dk/src/nrf53_bringup.c
index d9698045a2..c59768f9f8 100644
--- a/boards/arm/nrf53/nrf5340-dk/src/nrf53_bringup.c
+++ b/boards/arm/nrf53/nrf5340-dk/src/nrf53_bringup.c
@@ -285,6 +285,16 @@ int nrf53_bringup(void)
   usbdev_rndis_initialize(mac);
 #endif
 
+#ifdef CONFIG_NRF53_QSPI
+  /* Initialize the MX25 QSPU memory */
+
+  ret = nrf53_mx25_initialize();
+  if (ret < 0)
+    {
+      syslog(LOG_ERR, "ERROR: nrf53_mx25_initialize() failed: %d\n", ret);
+    }
+#endif
+
   /* Initialize BLE */
 
 #ifdef CONFIG_NRF53_APPCORE
diff --git a/boards/arm/nrf53/nrf5340-dk/src/nrf53_mx25.c b/boards/arm/nrf53/nrf5340-dk/src/nrf53_mx25.c
new file mode 100644
index 0000000000..6b5fb81570
--- /dev/null
+++ b/boards/arm/nrf53/nrf5340-dk/src/nrf53_mx25.c
@@ -0,0 +1,107 @@
+/****************************************************************************
+ * boards/arm/nrf53/nrf5340-dk/src/nrf53_mx25.c
+ *
+ * Licensed to the Apache Software Foundation (ASF) under one or more
+ * contributor license agreements.  See the NOTICE file distributed with
+ * this work for additional information regarding copyright ownership.  The
+ * ASF licenses this file to you under the Apache License, Version 2.0 (the
+ * "License"); you may not use this file except in compliance with the
+ * License.  You may obtain a copy of the License at
+ *
+ *   http://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS, WITHOUT
+ * WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.  See the
+ * License for the specific language governing permissions and limitations
+ * under the License.
+ *
+ ****************************************************************************/
+
+/****************************************************************************
+ * Included Files
+ ****************************************************************************/
+
+#include <nuttx/config.h>
+
+#include <debug.h>
+#include <stdio.h>
+#include <sys/types.h>
+
+#include <nuttx/fs/fs.h>
+#include <nuttx/mtd/mtd.h>
+#include <nuttx/spi/qspi.h>
+
+#include "nrf53_qspi.h"
+
+/****************************************************************************
+ * Public Functions
+ ****************************************************************************/
+
+/****************************************************************************
+ * Name: nrf53_mx25_initialize
+ *
+ * Description:
+ *   Initialize the MX25RXX QSPI memeory
+ *
+ ****************************************************************************/
+
+int nrf53_mx25_initialize(void)
+{
+  struct qspi_dev_s *qspi_dev;
+  struct mtd_dev_s  *mtd_dev;
+  char               blockdev[32];
+  int                ret = -1;
+
+  /* Create an instance of the NRF53 QSPI device driver */
+
+  qspi_dev = nrf53_qspi_initialize(0);
+  if (!qspi_dev)
+    {
+      _err("nrf53_qspi_initialize() failed\n");
+      return -1;
+    }
+
+  _info("nrf53_qspi_initialize() successful\n");
+
+  /* Use the QSPI device instance to initialize the MX25 device */
+
+  mtd_dev = mx25rxx_initialize(qspi_dev, true);
+  if (!mtd_dev)
+    {
+      _err("mx25rxx_initialize() failed\n");
+      return -1;
+    }
+
+  /* Configure the device with no partition support */
+
+  snprintf(blockdev, sizeof(blockdev), "/dev/mtdqspi%d", 0);
+
+  ret = register_mtddriver(blockdev, mtd_dev, 0755, NULL);
+  if (ret != OK)
+    {
+      _err("register_mtddriver() failed: %d\n", ret);
+      return -1;
+    }
+
+  _info("register_mtddriver() successful\n");
+
+#ifdef CONFIG_FS_LITTLEFS
+  ret = nx_mount(blockdev, "/mnt/qspi", "littlefs", 0, NULL);
+  if (ret < 0)
+    {
+      ret = nx_mount(blockdev, "/mnt/qspi", "littlefs", 0,
+                     "forceformat");
+      if (ret < 0)
+        {
+          _err("nx_mount() failed: %d\n", ret);
+        }
+      else
+        {
+          _info("nx_mount() successful\n");
+        }
+    }
+#endif
+
+  return ret;
+}